Leica LAS X Navigator from Leica Microsystems

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ItemLeica LAS X Navigator
CompanyLeica Microsystems
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Catalog NumberLAS X Navigator
QuantityEA
TypeImage Software

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

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Website: www.leica-microsystems.com

(1) SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelleNature CommunicationsNovember 30, 2023Liv Zimmermann, Xiaohan Zhao, Jana Makroczyova, Moritz Wachsmuth-Melm, Vibhu Prasad, Zach Hensel, Ralf Bartenschlager, Petr Chlanda(excitation wavelength: 540–580 nm, emission wavelength: 592–668 nm) covering 1.2 × 1.2 mm area using LAS X Navigator software (Leica). The final map was stitched using the Cryo-CLEM/Stitch TileScan plugin available at

(2) Quantification of ligand and mutation-induced bias in EGFR phosphorylation in direct response to ligand bindingNature CommunicationsNovember 21, 2023Daniel Wirth, Ece Özdemir, Kalina Hristovasettle for 1 h. Image acquisition was automated by selecting pre-defined regions and focus points in the LAS X Navigator software (Leica Biosystems, Wetzlar, Germany). Two scans (256×256 pixels) per image were acquired, a

(3) Expression profiles of the autism-related SHANK proteins in the human brainBMC BiologyNovember 13, 2023Sarah Woelfle, Maria T. Pedro, Jan Wagner, Michael Schön, Tobias M. Boeckersconfocal laser-scanning microscope (Leica, Wetzlar). Tilescans were acquired at 512 × 512 pixels using the LAS-X Navigator of the LAS-X software (Leica). Human brains For intensity quantification, first, a low-resolution

(4) Real-World Data on Combined EGFR-TKI and Crizotinib Treatment for Acquired and De Novo MET Amplification in Patients with Metastatic EGFR-Mutated NSCLCInternational Journal of Molecular SciencesAugust 23, 2023Edyta M. Urbanska, Morten Grauslund, Peter R. Koffeldt, Sarah L. B. Truelsen, Johan O. Löfgren, Junia C. Costa, Linea C. Melchior, Jens B. Sørensen, Eric Santoni-RugiuLeica DFC9000 camera incorporated with the microscope after identification of representative areas with the Leica LAS X Navigator Software, v.3.6.0 Widefield (Leica Microsystems, Brønshøj, Denmark). FISH was assessed by two readers

(5) Phospholipid scramblase Xkr8 is required for developmental axon pruning via phosphatidylserine exposureThe EMBO JournalJuly 17, 2023Urte Neniskyte, Ugne Kuliesiute, Auguste Vadisiute, Kristina Jevdokimenko, Ludovico Coletta, Senthilkumar Deivasigamani, Daina Pamedytyte, Neringa Daugelaviciene, Daiva Dabkeviciene, Emerald Perlas, et al.at 1.25 optical zoom, pixel size 0.144 × 0.144 μm2, z‐axial step size 1 μm, using Leica LasX SPE and LasX navigator software. Images were processed and analyzed using ImageJ (RRID:SCR_003070) software. Dendritic arborizations

(6) Inhibition of Fatty Acid Amide Hydrolase (FAAH) Regulates NF-kb Pathways Reducing Bleomycin-Induced Chronic Lung Inflammation and Pulmonary FibrosisInternational Journal of Molecular SciencesJune 14, 2023Tiziana Genovese, Andrea Duranti, Francesco Monaco, Rosalba Siracusa, Roberta Fusco, Daniela Impellizzeri, Ramona D’Amico, Marika Cordaro, Salvatore Cuzzocrea, Rosanna Di PaolaMasson trichrome, examined under a light microscope with a Leica DM6 attached to an imaging system (LasX Navigator), and graded by two researchers who were blind to the experimental groups. According to Ashcroft et

(7) Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial DamageToxicsMay 23, 2023Alessio Filippo Peritore, Gianluca Antonio Franco, Francesco Molinari, Alessia Arangia, Livia Interdonato, Ylenia Marino, Salvatore Cuzzocrea, Enrico Gugliandolo, Domenico Britti, Rosalia Crupimicroscope (Leica Microsystems SpA, Milan, Italy) equipped with a motorized stage and associated with the Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy) [37]. Histologic score was evaluated as described above

(8) Anatomical and functional maturation of the mid-gestation human enteric nervous systemNature CommunicationsMay 9, 2023Lori B. Dershowitz, Li Li, Anca M. Pasca, Julia A. Kaltschmidtmicroscope using a 20× (NA 0.75) oil objective. Acquisition areas were selected, acquired, and stitched with LASX Navigator Mode (Leica). Images were captured as z-stacks, which were set at 3 μm intervals capturing the full

(9) Trehalose–Carnosine Prevents the Effects of Spinal Cord Injury Through Regulating Acute Inflammation and Zinc(II) Ion HomeostasisCellular and Molecular NeurobiologyMay 1, 2023Irene Paterniti, Alessia Filippone, Irina Naletova, Valentina Greco, Sebastiano Sciuto, Emanuela Esposito, Salvatore Cuzzocrea, Enrico Rizzarelli2020) and evaluated using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) associated with Leica LAS X Navigator software using the objective lens at 10 × magnification (Leica Microsystems SpA, Milan, Italy). The

(10) MemophenolTM Prevents Amyloid-β Deposition and Attenuates Inflammation and Oxidative Stress in the Brain of an Alzheimer’s Disease RatInternational Journal of Molecular SciencesApril 8, 2023Daniela Impellizzeri, Mario Tomasello, Marika Cordaro, Ramona D’Amico, Roberta Fusco, Ali S. Abdelhameed, Uwe Wenzel, Rosalba Siracusa, Vittorio Calabrese, Salvatore Cuzzocrea, et al.sections under a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) with a motorized stage and Leica LAS X Navigator 7.31 software (Leica Microsystems SpA, Milan, Italy). 4.6. Western Blot Analysis Western blots on

(11) Quantifying organellar ultrastructure in cryo-electron tomography using a surface morphometrics pipelineJournal of Cell BiologyApril 3, 2023Benjamin A. Barad, Michaela Medina, Daniel Fuentes, R. Luke Wiseman, Danielle A. Grotjahnstacks with system optimized steps, GFP channel ex: 470, em: 525). Z stacks were stitched together in LAS X navigator to provide a single mosaic of maximum projections of the GFP signal, enabling rapid identification of

(12) Hypoxia-induced tracheal elasticity in vector beetle facilitates the loading of pinewood nematodeeLifeMarch 30, 2023Xuan Tang, Jiao Zhou, Tuuli-Marjaana Koski, Shiyao Liu, Lilin Zhao, Jianghua Sun561 nm lasers and have higher autofluorescence excitated by 561 nm laser. Images were processed using LAS X Navigator (Leica). RNA interference Double-stranded RNAs (dsRNAs) were synthesized with cDNA templates possessing

(13) Cytoplasmic contractile injection systems mediate cell death in StreptomycesNature MicrobiologyMarch 9, 2023Bastien Casu, Joseph W. Sallmen, Susan Schlimpert, Martin Pilhofer3D cell culture microscope (Leica) at room temperature. First, tile scan images were acquired on the LasX Navigator plug-in of Leica Application Suite X (LasX) software (v3.7.4.23463), and 100 targets were picked manually.

(14) Fisetin, a Natural Polyphenol, Ameliorates Endometriosis Modulating Mast Cells Derived NLRP-3 Inflammasome Pathway and Oxidative StressInternational Journal of Molecular SciencesMarch 7, 2023Alessia Arangia, Ylenia Marino, Roberta Fusco, Rosalba Siracusa, Marika Cordaro, Ramona D’Amico, Francesco Macrì, Emanuela Raffone, Daniela Impellizzeri, Salvatore Cuzzocrea, et al.(H&E) and evaluated using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) associated with Leica LAS X Navigator software 3.4.2. Histopathologic scores were evaluated as described previously [4]. Additionally, lesion

(15) Spermine oxidase induces DNA damage and sensitizes fusion negative rhabdomyosarcoma cells to irradiationFrontiers in Cell and Developmental BiologyJanuary 23, 2023Clara Perrone, Silvia Pomella, Matteo Cassandri, Michele Pezzella, Stefano Giuliani, Tecla Gasperi, Antonella Porrazzo, Anna Alisi, Anna Pastore, Silvia Codenotti, et al.reported in (Perrone et al., 2022). The images were taken using the Leica microscope Leica DMi8 with LAS X Navigator image acquisition software. Soft agar colony formation assays A total of 104 RD and JR1 cells, transduced

(16) Açai Berry Administration Promotes Wound Healing through Wnt/β-Catenin PathwayInternational journal of molecular sciencesJanuary 3, 2023Interdonato L, Marino Y, Franco GA, Arangia A, D'Amico R, Siracusa R, Cordaro M, Impellizzeri D, Fusco R, Cuzzocrea S, et al.examined using a Leica DM6 microscope (Leica Microsystems S.p.A., Milan, Italy) in association with the Leica LAS X Navigator software (Leica Microsystems S.p.A., Milan, Italy) [35]. 4.5. Immunohistochemical Localization of VEGF,

(17) Persistent muscle hyperalgesia after adolescent stress is exacerbated by a mild-nociceptive input in adulthood and is associated with microglia activationScientific ReportsOctober 31, 2022Sathish Kumar Singaravelu, Alexander Dawit Goitom, Akseli Petteri Graf, Handan Moerz, Andreas Schilder, Ulrich Hoheisel, Rainer Spanagel, Rolf-Detlef Treedeto get an overview of the entire L2 section (Fig. 1Bb left) with a computer-based imaging software LAS X Navigator (LEICA, Germany). For analysis of single cell morphology including all processes, three-dimensional

(18) UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcriptioniScienceOctober 1, 2022Declan L. Turner, Svenja Fritzlar, Sara Sadeghipour, Adele A. Barugahare, Brendan E. Russ, Stephen J. Turner, Rommel A. Mathiasfocus map was constructed with a single point per well and autofocused using the Hoechst channel in LAS X navigator (Leica). A 3 × 3 tilescan was performed per well in hoechst, and IE1 channels with 0% image overlap

(19) Açai Berry Mitigates Parkinson’s Disease Progression Showing Dopaminergic Neuroprotection via Nrf2-HO1 PathwaysMolecular NeurobiologyOctober 1, 2022Ramona D’Amico et al.light microscopy connected to an imaging system Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) with Leica LAS X Navigator software (Leica Microsystems SpA). [43]. Histological assessment was made by a blinded observer, and

(20) Chronic Exposure to Vinclozolin Induced Fibrosis, Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis in Mice KidneyInternational Journal of Molecular SciencesSeptember 25, 2022Davide Di Paola, Ramona Dâ€™Amico, Tiziana Genovese, Rosalba Siracusa, Marika Cordaro, Rosalia Crupi, Alessio Filippo Peritore, Enrico Gugliandolo, Livia Interdonato, Daniela Impellizzeri, et al.microscope (Leica Microsystems SpA, Milan, Italy) equipped with a motorized stage and associated with Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy) [68]. Histopathology scoring was applied as described

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Leica LAS X Navigator from Leica Microsystems

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